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. 2018 Apr 25;9:807. doi: 10.3389/fmicb.2018.00807

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Copyright © 2018 Shao, Abdel-Motaal, Chen, Chen, Xu, Meng, Zhang, Meng and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot detection and localization of RDD in Escherichia coli. Membrane fraction, cytoplasmic one and cell extract from E. coli KNabc/pET22b-PRO-RDD (Lanes 1, 3, 5) and KNabc/pET22b (Lanes 2, 4, 6) were sampled, respectively, and then subjected to SDS-PAGE (A) and western blot (B) analyses. The position of target protein RDD fused with an N-terminal His₆ tag is shown with a solid arrow.