Table 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Relevant phenotype or genotype | The source |
|---|---|---|
| Strains | ||
| Halobacillus andaensis NEAU-ST10-40T | Type strain of Halobacillus andaensis, a moderate halophile | Isolated and identified by our group (Wang et al., 2015) |
| Escherichia coli KNabc | ΔnhaAΔnhaBΔchaA | Donated by Professor Terry A. Krulwich (Nozaki et al., 1996) |
| Plasmids | ||
| pUC18 | Cloning vector | Takara Biotechnology (Dalian) Co. Ltd., China |
| pUC-SL38 | pUC18 carrying a 1145-bp DNA fragment with Na+/H+ antiport activity | This study |
| pET19b | Over-expression vector, AmpR | Novagen Ltd., United States |
| pET19b-truncated SppA | pET19 carrying 5′-end truncated SppA | This study |
| pET22b | Over-expression vector, AmpR | Novagen Ltd., United States |
| pET28b | Over-expression vector, KanR | Novagen Ltd., United States |
| pET28b-RDD | pET28b carrying the rdd gene fused in frame with an N-terminal His6 tag followed by a thrombin proteolytic cleavage site and a T7 tag | This study |
| pET22b-RDD | pET22b carrying the rdd gene fused in frame with an N-terminal His6 tag preceded by a T7 promoter | This study |
| pET22b-PRO-RDD | pET22b carrying the rdd gene fused in frame with an N-terminal His6 tag preceded by its native promoter instead of the T7 promoter | This study |