FIGURE 8:

The D667 region is necessary for interallelic complementation. (A) Assessing whether smc3-D667 complements the smc3-42 mutant. Haploid strains SMC3 (VG3486), smc3-42 (TE576), smc3-D667 (BRY467), smc3-42 smc3-D667 (BRY756), smc3-K113R (VG3486-K113R), and smc3-42 smc3-K113R (TE578) all contain the SMC3 URA3 CEN plasmid. Strains were grown to saturation in YPD cultures to allow loss of the SMC3 URA3 CEN plasmid and then played at 10-fold serial dilutions on YPD or FOA plates and incubated at the indicated temperatures. (B) Table summarizes interallelic complementation of haploid cells harboring the temperature-sensitive smc3-42 allele (Eng et al., 2015) and A.