FIGURE 2:

Adhesion frequency of pure Thy-1-Fc with supernatants with or without αvβ3-Fc integrin. Adhesion frequency was assessed by measuring the number of total binding events in at least 50 approach-retraction cycles per five pairs of freshly prepared beads. (A) Adhesion frequency of Thy-1-Fc measured in different dilutions of the supernatant containing the αvβ3-Fc protein (αvβ3-Fc) or the supernatant from cells transfected with mock plasmids (Mock Plasmid). (B) As a control for nonspecific interactions, adhesion frequency was also evaluated between Thy-1-Fc and the supernatant depleted of the αvβ3-Fc integrin (Depleted αvβ3-Fc) or with the HEPES buffer (Buffer), as well as between Thy-1(RLE)-Fc mutated in the integrin binding-site and the supernatant containing the αvβ3-Fc (αvβ3-Fc). The binding frequency between the two types of beads was also tested (Buffer/Buffer). Data are expressed as the mean ± SEM (*p < 0.05; **p < 0.01; n.s., nonsignificant).