Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 1;29(3):270–284. doi: 10.1091/mbc.E17-09-0561

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Lam et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 8: — A PKC is activated by LLO. HepG2 cells expressing MyrPalm-CKAR were imaged on the microscope stage at 37°C. Phase-contrast (PC) and fluorescence images (I_A, I_D, and I_F) were acquired every 20 s for 20 min. LLO was added after 5 min of imaging (T₀ and black vertical line). (A) A representative cell exhibiting LLO-induced MyrPalm-CKAR phosphorylation. From left to right: PC, overlay of PC + IA, I_A, I_D, and I_F, and FRET (in pseudo-color scale). From top to bottom: time points 0, 1, and 5 min after addition of LLO. Scale bar = 10 μm. (B) Data are presented as the average fold change in E_AVE ± SEM (n = 3, 24 cells in DMSO control, 26 cells in GF109203x, 28 cells in DMSO +LLO, and 26 cells in GF109203x +LLO).