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Rac1 is activated downstream of a conventional PKC.
| Toxin | — | LLO | LLO |
|---|---|---|---|
| Medium | DMSO | DMSO | GF109203x |
| Time (min) | 20 | 20 | 20 |
| NC | 115 | 92 | 136 |
| FRET+ cells | 1 | 50 | 26 |
| % FRET | 0.87 | 54.3 | 19.12 |
Cells were exposed to DMSO (0.2%), 0.1 nM LLO, or GF109203x. Time indicates the total duration of imaging. NC is the number of analyzed cells. FRET+ cells is the number of FRET-positive cells (exhibiting at least a twofold increase in FRET). % FRET is the percentage of FRET-positive cells (n > 3).
