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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Cancer Genet. 2018 Apr 9;224-225:21–28. doi: 10.1016/j.cancergen.2018.03.002

Figure 1. Optimization of BAP1 immunostaining and representative of tumors with negative and positive staining.

Figure 1

A) Two cell lines OCM3 (BAP1 positive) and NCI-H226 (BAP1 negative) were selected. Western blot confirmed BAP1 nuclear expression in OCM3. Immunostaining showed strong nuclear expression in OCM3 but no expression in the NCI-H226 cell line.

B) A cholangiocarcinoma case with loss of BAP1 expression occasional nuclear expression is seen in some ducts which are possibly non tumorous. Stroma and blood vessels endothelial cells are positive for nuclear BAP1 expression.

C) A cholangiocarcinoma with preserved nuclear expression of BAP1. Weak cytoplasmic expression is also observed.