Table 1.

Studies using siRNA-targeted delivery as treatment strategies in oral cancer

Targeted gene	Expression	Delivery system	Cell line	Animal model	Methods	Effect on	Reference
CIP2A	Up	599 peptide (viroporins) + Lipofectamine	CAL 27 and SCC-25	–	Agarose gel shift assay, fluorescence microscopy, RT-PCR, Western blot, CellTiter kit for cell proliferation, CytoSelect 24-well cell invasion assay kit, anchorage-independent growth assay	Regulate anchorage- independent growth and inhibition of invasion	42
Cip2A	Up	EGFR-binding peptide and endosome-disruptive peptide	CAL 27 and SCC-15	Nude mice	Cell proliferation assay, hydrodynamic diameter and zeta potential measurement, BIO-RAD ChemiDoc XRS system	Prevent invasion and tumor growth	28
CxcR4	Up	Lentiviral vectors	SCC-9, SCC-4, and Tca8113 cell lines	Nude mice	RT-PCR, Western blot, propidium iodide staining for cell cycle, Annexin V for apoptosis, CCK8, IHC, and microarray	Regulate cell cycle and proliferation	67
VEGF	Up	Folic acid-decorated polyamidoamine dendrimer G4	HN-12	Female athymic nude mice	ELISA, qRT-PCR, tumor size, H&E staining	Inhibit angiogenesis	68
MDR1 and MDR2	Up	Adeno-associated viral vector	KB	No	RT-PCR, Western blot, flow cytometry, MTT assay	Regulate MDR	51
MDR1	Up	Polymer polyethylenimine	Doxorubicin	KBV cells, BALB/c nude mice	Cytotoxicity assays in vitro, drug loading rate and release assays, agarose gel retardation assay, qRT-PCR, apoptosis by flow cytometry	Regulate MDR	69

Abbreviations: CCK8, cell counting kit 8; EGFR, epidermal growth factor receptor; ELISA, enzyme-linked immunosorbent assay; IHC, immunohistochemistry; MDR, multidrug resistance; KBV, oral squamous cells; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; siRNA, small interfering RNA.