Figure 2.

BCFH-DMR in an alternate promoter of ESR1. Map and bis-seq showing the BCFH-DMR in an alternate promoter of ESR1. Zoomed-in view of ESR1, showing relevant ENCODE tracks and the amplicons utilized for targeted bis-seq. The BCFH-DMR is located in a downstream ESR1 alternative promoter region and marked by poised chromatin marks. Graphical representation of bis-seq data for 10 examples of BCFH- girls (left) and BCFH+ girls (right) are shown. Each column represents a consecutive CpG covered by the amplicon, and each line a unique DNA fragment. White circles are unmethylated CpGs and black circles are methylated CpGs.