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. Author manuscript; available in PMC: 2018 Jun 12.
Published in final edited form as: Nature. 2014 Dec 3;518(7540):547–551. doi: 10.1038/nature13989

Extended Data Figure 8. Analysis of fetal stem/progenitor cells and fetal macrophages in Tie2MeriCreMer Rosa26YFP embryos pulse-labelled from E6.5 to E10.5.

Extended Data Figure 8

a, Experimental design for fate mapping analysis of Tie2MeriCreMerRosa26YFP embryos pulse-labelled at E6.5, or E7.5, or E8.5, or E9.5 or E10.5. b, c, Representative flow cytometry of fetal liver stem/progenitor cells (b) and of fetal macrophages (c) in the yolk sac, head region, and embryo body at E12.5, injected at E6.5 or at E10.5. d, Representative images of May-Grünwald-Giemsa stained cytospin preparations of sorted YFP+ and YFP- CD45+ F4/80bright macrophages of the embryo proper or the head region of E13.5 Tie2MeriCreMerRosa26YFP embryos pulsed at E7.5. Scale bar, 10μm e, Quantification of the percentage of YFP+ stem/progenitor cells in the fetal liver and macrophages in yolk sac, brain (head) and embryo body at E12.5. Embryos were labelled at E6.5 (n=5), or E7.5 (n=7), or E8.5 (n=4), E9.5 (n=5) or E10.5 (n=7) and analysed at E12.5 (mean±s.d.).