Skip to main content
. Author manuscript; available in PMC: 2018 Jun 12.
Published in final edited form as: Nature. 2014 Dec 3;518(7540):547–551. doi: 10.1038/nature13989

Figure 4. Fetal macrophages and adult tissue-resident macrophages originate from Tie2-expressing progenitors prior to E10.5.

Figure 4

a, Fate mapping analysis of Tie2-expressing cells after tamoxifen (TAM) administration at E7.5, or E8.5, or E9.5 or E10.5. Arrows indicate time points for analysis. b, Flow cytometric analysis of fetal liver long-term or short-term hematopoietic stem cells (LT-HSCs, ST-HSCs), multipotent progenitors (MPPs), common myeloid progenitors (CMPs), granulocyte-monocyte progenitors (GMPs), megakaryocyte-erythrocyte progenitors (MEPs) (left panel) and of fetal macrophages (right panel) in the yolk sac, brain, and fetal liver. Time points of labelling (E7.5 (n=7); E10.5 (n=7)) and analysis are indicated, and for each experiment one representative analysis is shown. See Extended Data Fig. 8 for quantitative analysis. c, Frequencies of labelled HSCs and progenitor cells, splenocytes, and F4/80low CD11bhigh myeloid cells and F4/80bright resident macrophages in spleen, liver lung, epidermis and brain were analysed (mean ± s.d.) from 6-8-week-old Tie2MeriCreMer animals pulse-labelled at E7.5 (n=4), E8.5 (n=4), E9.5 (n=4) or E10.5 (n=6).