Figure 3.

Upregulation of HES1 expression could promote Doxorubicin-induced apoptosis.

Notes: Cells were stably transfected with HES1 overexpression plasmids. Cells transfected with empty pOZ vector were used as the control group. (A) The overexpression efficiency of HES1 was measured by real-time RT-PCR. (B) The levels of the overexpressed protein were detected by Western blot. (C) The cell viability of overexpressed cell line was detected by MTT. (D) The overexpressed cells were stained with Multicaspase/7-AAD for quantitative measurement by using flow cytometry. (E) Bars represent the mean of three independent experiments performed in triplicate of (D). ^#P>0.05, **P<0.01. The cells were treated with Doxorubicin (300 μM) for 24 hours.

Abbreviations: 7-AAD, 7-aminoactinomycin D; RT-PCR, reverse transcriptase polymerase chain reaction.