Figure 4.

Downregulation of HES1 expression could inhibit Doxorubicin-induced apoptosis.

Notes: Cells were stably transfected with HES1 shRNA plasmids. Cells transfected with empty pLko.1 vector were used as the control group. (A) The knockdown efficiency of HES1 was measured by real-time RT-PCR. (B) The cell viability of knockdown cell lines was detected by MTT. (C) The knockdown and control cells were treated with Doxorubicin (300 μM) for 24 hours and then stained with Multicaspase/7-AAD for the quantitative measurement by using flow cytometry. (D) Bars represent the mean of three independent experiments performed in triplicate of (C). **P<0.01. ^#P>0.05.

Abbreviations: 7-AAD, 7-aminoactinomycin D; RT-PCR, reverse transcriptase polymerase chain reaction.