Figure 5.

HES1 regulates apoptosis by activating PARP1.

Notes: HeLa cells were pretreated with PARP1 inhibitor Rucaparib (1 nM) for 7 days, and then, the cells were treated with Doxorubicin (300 μM) for 24 hours. (A) Cell apoptosis of HES1-knockdown cell lines was determined by flow cytometry. (B) Bars represent the mean of three independent experiments performed in triplicate of (A). (C) Cell apoptosis of HES1 overexpressing cell line was determined by flow cytometry. (D) Bars represent the mean of three independent experiments performed in triplicate of (C). **P<0.01, *P<0.05, ^#P>0.05.

Abbreviation: PARP1, poly(ADP-ribose) polymerase-1.