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. 2018 Jun 11;6(11):e13721. doi: 10.14814/phy2.13721

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© 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A and B) MIP‐2α and IL‐6 protein secretion are reduced when CD11b⁺ macrophages are depleted. In vitro assay of secreted MIP‐2α and IL‐6 protein were both significantly reduced in F480⁺ macrophages isolated from CD11b^DTR mice treated with diphtheria toxin (DT) (DTR + DT) compared with CD11b^DTR mice treated with PBS (DTR + PBS). Cytokines were normalized for total cell protein (pg/mg cell protein). *P < 0.05.