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. Author manuscript; available in PMC: 2019 Apr 16.
Published in final edited form as: Mol Omics. 2018 Apr 16;14(2):121–133. doi: 10.1039/c7mo00064b

Figure 2.

Figure 2

K-BIPS study with OA. (A) Avidin purification of biotinylated proteins after kinase-catalyzed biotinylation. Biotinylation was carried out with lysates from HeLa cells untreated (ethanol only; lane 1) or treated with OA (1 or 0.01 μM in ethanol; lanes 2–3). After reaction, biotinylated proteins were enriched with avidin resin (elution in lanes 4–6). Proteins in each sample were separated by SDS-PAGE and stained for total proteins using SYPRO® Ruby stain. Full gel image shown in Figure S1. (B) Known substrates identified by K-BIPS (Substrate), along with the corresponding PPP family member. Refer to Table S1 for primary literature. (C) Phosphatase interacting proteins (Gene name) from the K-BIPS hits. Interaction information was obtained from Uniprot and BioGrid databases, or literature (see Table S1). The catalytic and regulatory subunits known to interact with the hit are indicated. For example, PPP1CA refers to PP1 alpha isoform of the catalytic subunit, whereas PPP1R8 refers to the regulatory subunit 8 of PP1 phosphatase.