FIG. 2.

Contact guidance in 2D is cell cycle-dependent. (a) and (b) Representative trajectories of cells in G1 (red) and S/G2 (green) phases, randomly migrating on gelatin-coated plates. (c) and (d) Persistence and instantaneous velocity of cells shown in (a) and (b). (e) and (f) Representative trajectories of cells in G1 (e) and S/G2 (f) phases of the cell cycle migrating inside the gelatin-coated microchannels in the presence of the EGF gradient. (g) and (h) Persistence and instantaneous velocity of cells shown in (e) and (f). (i) and (j) Representative trajectories of cells in G1 (i) and S/G2 (j) phases of the cell cycle migrating inside the gelatin-coated microchannels, in the absence of the EGF gradient. (k) and (l) Persistence and instantaneous velocity of cells shown in (i) and (j). (m) and (n) Representative trajectories of cells monitored continuously as they migrate in G1and S/G2, either randomly (m) or contact guided, in microchannels (n). (o) and (p) G1 to S/G2 persistence ratio (o) and instantaneous velocity ratio (p) for randomly migrating (R) and contact guided cells (CGs). In all box and whisker plots, solid and dotted lines represent the median and mean, respectively.