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. 2018 May 30;2(3):031904. doi: 10.1063/1.5026419

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© 2018 Author(s).

2473-2877/2018/2(3)/031904/13

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FIG. 4. — 3D contact guidance is cell cycle-dependent. (a) and (b) Representative trajectories of cells in the G1 (red) and the S/G2 (green) phase of the cell cycle migrating in 3D collagen with randomly oriented fibers. (c) and (d) Persistence and instantaneous velocity of cells migrating in 3D collagen with randomly oriented fibers. (e) and (f) Representative trajectories of cells in the G1 (red) and the S/G2 (green) phase of the cell cycle migrating in aligned 3D collagen fibers. (g) and (h) Persistence and instantaneous velocity of cells directionally migrating in aligned 3D collagen fibers. In all box and whisker plots, solid and dotted lines represent the median and mean, respectively.