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. 2018 Jun 12;13(6):e0199173. doi: 10.1371/journal.pone.0199173

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© 2018 Fonseca-Alves et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Double immunohistochemical staining for p63 and CK8/18. (A) PC with p63-/CK8/18+ phenotype. It is possible to note cytoplasmic CK8/18 expression (red staining—arrow) in neoplastic cells and nuclear negative p63 staining in neoplastic cells. There is a p63+/CK8/18- basal cell (arrowhead) (internal control). Negative signal can be observed as the blue color. (B) PC with p63+/CK8/18+ phenotype. Note the neoplastic doubled stained cells (arrows). It is also observed p63-/CK8/18+ cells (arrowhead) and p63+/CK8/18- basal cells (doted circle).