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. 2018 Jun 12;13(6):e0198359. doi: 10.1371/journal.pone.0198359

Table 1. Antibodies used for FACS experiments.

Cells Primary antibody Conjugate Host species Dilution
Pan-leukocyte marker CD45 PerCP/Cy5.5 Mouse 1:400
Pan-T cell marker CD3 PerCP/Cy5.5 Mouse 1:400
T cell receptor TCRβ APC Rat 1:250
Granulocytes GR-1, CD11b PE-Cy7 Rat 1:100
Cytotoxic T cells CD8 Pacific Blue Rat 1:100
Helper T cells CD4 Pacific Orange Rat 1:100
B cells B220 FITC Mouse 1:400
Macrophages CD11b, Ly6C, Ly6G, CD206, F4/80 PE Rat 1:200
Dendritic cells CD11c Pacific Blue Rat 1:250
Major histocompatibility complex II MHC-II Brilliant Violet 510 Rat 1:800
Eosinophils CD193 Alexa Fluor 647 Rat 1:200
NK cells CD49b PE Rat 1:100
γδ T cells γδ-TCR FITC Mouse 1:500
NKT cells CD1d α-Galactosylceramide tetramer PE Rat 1:500