Table 1. Antibodies used for FACS experiments.
Cells | Primary antibody | Conjugate | Host species | Dilution |
---|---|---|---|---|
Pan-leukocyte marker | CD45 | PerCP/Cy5.5 | Mouse | 1:400 |
Pan-T cell marker | CD3 | PerCP/Cy5.5 | Mouse | 1:400 |
T cell receptor | TCRβ | APC | Rat | 1:250 |
Granulocytes | GR-1, CD11b | PE-Cy7 | Rat | 1:100 |
Cytotoxic T cells | CD8 | Pacific Blue | Rat | 1:100 |
Helper T cells | CD4 | Pacific Orange | Rat | 1:100 |
B cells | B220 | FITC | Mouse | 1:400 |
Macrophages | CD11b, Ly6C, Ly6G, CD206, F4/80 | PE | Rat | 1:200 |
Dendritic cells | CD11c | Pacific Blue | Rat | 1:250 |
Major histocompatibility complex II | MHC-II | Brilliant Violet 510 | Rat | 1:800 |
Eosinophils | CD193 | Alexa Fluor 647 | Rat | 1:200 |
NK cells | CD49b | PE | Rat | 1:100 |
γδ T cells | γδ-TCR | FITC | Mouse | 1:500 |
NKT cells | CD1d α-Galactosylceramide tetramer | PE | Rat | 1:500 |