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. 2018 May 29;7:e37084. doi: 10.7554/eLife.37084

Figure 3. FBXL19 recruits CDK-Mediator to chromatin.

(A) A schematic representation of the integration site of the TetO array. (B) ChIP-qPCR analysis of the binding of the indicated proteins across the TetO array in TetR only (top) and TetR-FBXL19 (bottom) ES cell lines. The x-axis indicates the spatial arrangement of the qPCR amplicons with respect to the center of the TetO array (in kb). Error bars represent SEM of three biological replicates.

Figure 3.

Figure 3—figure supplement 1. FBXL19 recruits CDK-Mediator to chromatin.

Figure 3—figure supplement 1.

(A) Western blot analysis of the expression of the TetR-fusion proteins from nuclear extracts of the respective cell lines. BRG1 was probed as a loading control. (B) ChIP-qPCR analysis of the binding of the indicated proteins across the TetO array (distance from center shown) in TetR-KDM2A (top) and TetR-KDM2B (bottom) ES cell lines. The x-axis indicates the spatial arrangement of the qPCR amplicons with respect to the center of the TetO array (in kb). Error bars represent SEM of three biological replicates.