(A) Examples of hindlimb innervation patterns and distribution of ACh nicotinic receptors in a whole-mount hindlimb bud, revealed by fluorescence immunolabeling of neurofilament associated protein (Neurofil., red), glutamate receptor (NR2b, blue) and α-bungarotoxin labeling (α-bungaroTx, yellow) respectively, at stages 52 (upper panels) and 57 (lower panels). Inset drawings at bottom left of each panel show bud morphology at the two representative larval stages. Scale bars = 200 µm for stage 52, 100 µm for stage 57. (B) Examples of fluorescence immunolabeling against neurofilament associated protein (red), synaptophysin (Synaptoph., green) and NMDA glutamate receptor subunit 1 (NR1, blue) in whole-mount limb bud at stage 52. White arrowheads indicate sites of apposition of all three markers. Scale bar = 20 µm. (C) Examples of fluorescence immunolabeling against neurofilament associated protein (red), the glutamate vesicular transporter 1 (VGluT1, yellow) and the glutamate receptor subunit NR2b (blue) in whole-mount limb at stage 52. Scale bar = 50 µm.