. Author manuscript; available in PMC: 2018 Jun 12.

Published in final edited form as: Dev Neurobiol. 2016 Sep 21;77(6):708–725. doi: 10.1002/dneu.22448

Table 1.

Custom Primer Sets for MSP Analysis (Lubin et al., 2008; Roth et al., 2009, 2015)

Primer	Sequence (5′ to 3′)
Bdnf exon I Methylated	Forward: CGGAAAGTATTAGAGGTAGGGTAGC
Bdnf exon I Methylated	Reverse: TACGAACCCTAAATCTCTAAACGAA
Bdnf exon I Unmethylated	Forward: TGGAAAGTATTAGAGGTAGGGTAGTGA
Bdnf exon I Unmethylated	Reverse: TACAAACCCTAAATCTCTAAACAAA
Bdnf exon IV Methylated	Forward: GGTAGAGGAGGTATTATATGATAGTTTACG
Bdnf exon IV Methylated	Reverse: TAAATAAAAAAAACGACAACGCGAA
Bdnf exon IV Unmethylated	Forward: AGGTAGAGGAGGTATTATATGATAGTTTAT
Bdnf exon IV Unmethylated	Reverse: TAAATAAAAAAAACAACAACACAAA
*Tubulin*	Forward: GGAGAGTAATATGAATGATTTGGTG
*Tubulin*	Reverse: CATCTCAACTTTCCCTAACCTACTTAA