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. 2018 Jan 5;5(2):80–85. doi: 10.1021/acs.estlett.7b00495

Figure 1.

Figure 1

(A) Bovine cleaved embryo and blastocyst production efficiency displayed as percentages of the total number of mature cumulus–oocyte complexes (COCs) submitted for in vitro fertilization (IVF). IVF and in vitro culture (IVC) were performed in the presence of PIC100, E-Shell200, E-Shell300, PDMS, or PS polymer structures. Standard IVF and IVC were also performed without polymer structures (Control). (B) BG1ERE bioassay to detect increases in ER-dependent luciferase activity caused by media conditioned with PIC100, E-Shell-200, E-Shell300, PS, or PDMS, or without any conditioning. An increase in luciferase intensity was found for PIC100, E-Shell-200, E-Shell300, and PDMS; 1 and 10 pM estrogen (E2) were used to check the responsiveness of the cells in the assay. Different asterisks and number signs indicate statistically significant differences (p < 0.05).