Skip to main content
. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Brain Struct Funct. 2018 Mar 23;223(6):2685–2698. doi: 10.1007/s00429-018-1652-y

Figure 5. CRF neurons monsynaptically project to CRFR1+ and Npas1+ GPe neurons.

Figure 5

In animals traced from CRFR1+ neurons in the GPe (A–C) we found monosynaptically connected neurons in the lateral-dorsal Bed Nucleus of the Stria Terminalis (BSTld; A), the Paraventricular nucleus of the hypothalamus (PVN; B) and the Central Nucleus of the amygdala (CeA; C). Staining for CRF (second column; green) reveals that a portion of GPe projecting neurons are CRF positive. Arrows indicate CRF+ GPe projecting neurons, arrowheads indicate CRF− GPe projecting neurons. In Npas1+ GPe tracing experiments we also saw monosynaptically projecting neurons in the BSTld (D), PVN (E) and CeA (F). Staining of sections for CRF (green) show that some of these neurons contain CRF peptide (Magenta, arrows), while other neurons do not (arrowheads). A–L, Scalebar = 50µm. (H) Quantification of the percentage of neurons that are CRF positive in each stress-related nucleus. Of the sections that were stained for CRF, we found the ratio of neurons listed above each bar were positive for CRF peptide. (G) Quantification of the connectivity between key stress responsive nuclei and CRFR1 neurons (red) and Npas1 neurons (blue) in the GPe. The CeAl (lateral division of the CeA) and CeAm (medial division of the CeA) make more connections with Npas1+ neurons than with CRFR1+ neurons. * = p<0.05, n=3 per genotype. Connectivity ratio is the number of neurons present in each nucleus per starter neuron.