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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Dev Biol. 2018 May 19;440(2):129–136. doi: 10.1016/j.ydbio.2018.05.008

Figure 6. Wnt signaling inhibits Map3k1 promoter.

Figure 6

HEK293 cells were co-transfected with CMV-renilla luciferase reporter and (A) Wnt/β-catenin signaling luciferase reporter in its wild type (TCF/LEF-luc) or mutant (TCF/LEF-mut-luc) forms, and (B) Map3k1 promoter driven luciferase reporter (pMap3k1-luc), together with either empty vector or Wnt3a expression plasmids. At 48 h after transfection, luciferase activities were examined. In some experiments, the relative luciferase activity was calculated using firefly-luciferase activity versus renilla-luciferase activity, and the value in control cells was set as 1. The statistical significance was determined using Student t-test, **P<0.01 and ***P<0.001 are considered significant.