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a Ion exchange chromatography of DEAE-Sepharose FF (30 × 1.6 cm, i.d); b zymogram of neutral protease activity; c SDS-PAGE analysis of crude and purified protease from Aspergillus oryzae Y1. Lane 1 low molecular weight; lane 2 crude extract; lane 3 ammonium sulfate precipitation; lane 4 purified neutral protease
