Fig. 4.

a Effect of temperature on protease activity; b stability of temperature on purified protease activity was determined at different incubation times (0–60 min) at different temperatures at pH 7.0. The residual protease activity was estimated under the enzyme assay conditions and indicated as the percentage relative to the activity of the untreated protease activity; c stability of temperature on commercial neutral protease activity was determined at different incubation times (0–60 min) at different temperatures at pH 7.0. The residual protease activity was estimated under the enzyme assay conditions and indicated as the percentage relative to the activity of the untreated protease activity