Table 1.

Primer sequences

Gene symbol	Gene bank accession number	Primer pair (fwd/rev)	Amplicon size (bp)
Col1a1	NM_007742.3	5′- CCGGAAGAATACGTATCACCA-3′ 5′- TCTGGGAAGCAAAGTTTCCT-3′	199
Col1a2	NM_007743.3	5′- CTTACTGGGAACTTTGCTGCTC-3′ 5′- TTTCCAGGGTGACCATCCTC-3′	229
Spp1	NM_001204201.1	5′- GGAAACCAGCCAAGGTAAGC-3′ 5′- TGCCAATCTCATGGTCGTAG-3′	92
Runx2	NM_001271627.1	5′- AGGGACTATGGCGTCAAACA-3′ 5′- GGCTCACGTCGCTCATCTT-3′	137
Bglap2	NM_001032298.3	5′- CACCTAGCAGACACCATGAG-3′ 5′- TGGACATGAAGGCTTTGTCAG-3′	123
Ibsp	NM_008318.3	5′- GGTCTTTAAGTACCGGCCAC-3′ 5′- CGTTTGAAGTCTCCTCTTCCTC-3′	157
Alpl	NM_001287172.1	5′- GCAACTCCATCTTTGGTCTG-3′ 5′- GTTGTTGTGAGCGTAATCTACC-3′	145
Nog	NM_008711.2	5′- GCCAGCACTATCTACACATCC-3′ 5′- GCGTCTCGTTCAGATCCTTCTC-3′	114
Gapdh	NM_008084.2	5′-CTTGTGCAGTGCCAGCCTC-3′ 5′-GCCGTGAGTGGAGTCATACTG-3′	189