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. 2018 Jun 12;8:8963. doi: 10.1038/s41598-018-27306-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR quantification of selected genes of interest in the SCG: Gene expression was assessed in the SCG as an alternative sympathetic ganglion. Relative gene expression levels expressed as a ratio to Gapdh expression in left SCG (Mean ± SEM, n = 6 M vs 4 F, n = 3 F for Ret). The gene expression levels of Ntng1, Ret and Tcfl5 were much lower in the SCG compared to the stellate. The sex differences for Slc18a1, Ret and Ntng1 only were present in both the stellate and SCG. *p < 0.05, **p < 0.01, ***p < 0.001.