Skip to main content
. 2018 Jun 12;8:8989. doi: 10.1038/s41598-018-27224-4

Figure 2.

Figure 2

ARB causes dose-dependent inhibition of ZIKV protein synthesis. Vero cells were treated with ethanol solvent control (E) or 3.8, 7.5, 15, or 30 μM of ARB for 30 minutes prior to infection with ZIKV MR766 at an MOI of 0.01. The images on the left side of the figure depict ZIKV Envelope (E), NonStructural 1 (NS1), and Capsid protein expression, detected by Western blot analysis of protein lysates harvested 72 hours post-infection. The image is a composite of the following antibody probings: the blot was first probed with rabbit anti-ZIKV-E (detected with anti-rabbit 800 labeled secondary antibodies) and mouse anti-vinculin antiserum (detected with anti-mouse 680 labeled secondary antibodies). The blot was stripped and reprobed with rabbit anti-ZIKV-Capsid (detected with anti-rabbit 800 labeled secondary antibodies) and mouse anti-ZIKV-NS1 antiserum (detected with anti-mouse 680 labeled secondary antibodies). M = mock infected cells. No Rx = ZIKV-infected cells without further treatment. E = ZIKV-infected cells treated with ethanol solvent control. Molecular weight markers are as indicated. Right bar graph shows the relative pixel intensity across the 4 doses of ARB used in this assay. Original blot images are shown in Supplemental Information.