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. 2018 Jun 12;8:8989. doi: 10.1038/s41598-018-27224-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ARB inhibits ZIKV in A549 cells. Cells were pretreated with various doses of ARB either 1 hour before ZIKV infection (RIGHT) or overnight (LEFT) prior to infection with ZIKV MR766 (MOI = 1). Virus was added to cells in the presence of drug, and two-hours later, viral inocula were removed. Medium containing fresh ARB at the respective doses was then added back to cells. Infection was monitored 48 h post infection by immune-staining cells with pan flavivirus anti-E protein antibody 4G2. The expression of protein antigen was quantified using anti-mouse HRP-TMB method by measuring absorbance at 650 nm. Viability was measured by CellTiter-Glo reagent (Promega). The solvent control (i.e. ethanol) was set at 100% for the infection and viability assessments and is reflected on the Y-axis. Data are means ± standard deviation of three replicates.