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. 2018 Jun 12;8:8981. doi: 10.1038/s41598-018-27346-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Composition of the nuclear lamina is stiffness-responsive in primary and immortalised MSCs. (a) Immunofluorescence imaging was used to quantify lamins -A/C and -B1 (LMNA and LMNB1) in primary and immortalised MSCs cultured on soft (2 kPa) or stiff (25 kPa) collagen-I coated PA hydrogels. Lamin-B1 expression appeared constant in all conditions, consistent with earlier reports¹⁵. (b) Quantification of lamin A/C to B1 ratio (LMNA:LMNB1) from immunofluorescence images showed that lamin-A/C was consistently upregulated on stiff substrates, relative to lamin-B1, in each of four primary donors and the immortalised MSCs (N indicates number of cells analysed per condition). (c) LMNA:LMNB1 was significantly increased on stiff substrates (p-values from paired t-tests and Kruskal-Wallis (KW) tests as indicated; n = 4 primary donors).