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. 2018 Jun 12;8:8981. doi: 10.1038/s41598-018-27346-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mechanosensitive features of primary MSCs maintained in the immortalised MSC line. (a) Mechano-regulatory pathways observed in both primary and immortalised cells included: modulation of cell spreading and morphology, regulation of the nuclear lamina, translocation of transcription factors YAP1 and MKL1, and changes to differentiation potential. (b) The maintained sensitivity of YAP1 location to substrate stiffness enabled development of a reporter cell line: cytoplasmic GFP-YAP was indicative of a soft substrate, while colocalisation with nuclear RFP-H2B indicated a stiff substrate.