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. 2018 Jun 12;8:8951. doi: 10.1038/s41598-018-27179-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The characterization of gradient generation in the MPOC platform. The effect of (a) flow rate and (b) diffusion coefficients at a flow rate of 30 µL/hr on a gradient profile by COMSOL^® Multiphysics. The effect of (c) a flow rate and (d) diffusion coefficients at a flow rate of 30 µL/hr on a gradient profile by an experiment using fluorescently-labeled solution with different molecular weight (n = 3, standard error of the mean (SEM) <±7.0).