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. 2018 Jun 12;8:8987. doi: 10.1038/s41598-018-27220-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RCC4-EV cells are more resistant to propofol-induced cell injury than RCC4-VHL cells. RCC4-VHL and RCC4-EV cells were exposed to the indicated propofol concentrations for 6 h. (a) Cells were harvested and cell death was detected by flow cytometry. The ratio of annexin V- and/or PI-positive positive cells [(Q1 + Q2 + Q4)/(Q1 + Q2 + Q3 + Q4)] was used to calculate the percentage of dead cells. (b) The cell death are shown for each treatment group (n = 3). *p < 0.05, as compared to the control cell population (no treatment); ^#p < 0.05, for the indicated comparison.