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. 2018 Jun 12;8:8987. doi: 10.1038/s41598-018-27220-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reprogrammed oxygen metabolism in RCC4-EV cells. OCR (a) and ECAR (b) in the indicated cells under normoxic (20% O₂) conditions. (c) Cell Mito Stress test™ profile of the key parameters of mitochondrial oxygen consumption rate (OCR). The values of basal OCR (d), maximal OCR (e), the non-mitochondrial respiration rate (f), and proton leakage (g) are also indicated for RCC4-EV and RCC4-VHL cells. (h) Equal numbers of RCC4 and RCC4- VHL cells were stained with MitoTracker™ Green FM and analyzed by flow cytometry to measure mitochondrial mass. Differences between results were evaluated by t-test ^#p < 0.05 compared to the control cell population (group).