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. 2018 Jun 12;8:8987. doi: 10.1038/s41598-018-27220-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Forced HIF-1 activation is sufficient to resist propofol toxicity in SH-SY5Y neuroblastoma cells. (a) SH-SY5Y cells were incubated with (+) or without (−) 200 µM nPG or 1 mM DMOG for 4 h. 35 µg of whole-cell lysates were immunoblotted (IB) using primary antibodies raised against the indicated proteins. (b) OCR and ECAR were measured in SH-SY5Y cells exposed to 100 µM nPG or 100 µM DMOG for 4 h. (c and d) SH-SY5Y cells were exposed to 100 µM nPG and 100 µM DMOG for 4 h and then exposed to 50 µM propofol for 6 h. Graphic depictions of caspase-3/7 activity (n = 3) (c) and cell death (n = 3) (d). Differences were evaluated by two-way ANOVA followed by Dunnett’s test for multiple comparisons; *p < 0.05, as compared to the control cells (no treatment); ^#p < 0.05 for the indicated comparison.