Figure 2.

Vav-H2B-Dendra2 (VHD) transgenic mice allow tracking of lymphocytes for at least 28 days after photoconversion. (a) Expression of H2B-Dendra2 protein (D-Green) in CD19⁺ B cells, αβ CD4⁺ T cells, αβ CD8⁺ T cells and γδ T cells from a VHD mouse (green histograms) and αβ CD8⁺ T cells from a WT mouse (gray histogram) (representative of >50 mice in >10 experiments). (b) In vitro proliferation of ex vivo photoconverted (right) and nonconverted (left) αβ CD8⁺ T cells from VHD mice that are labelled with eFluor 450 proliferation dye. Blue histograms show unstimulated cells (representative of 4 experiments). (c and d) Frequency of D-Red⁺ cells among αβ CD8⁺ (c) and γδ (d) T cells immediately, 12 hours, 7 days or 28 days after photoconversion of inguinal LN in VHD mice (representative of ≥2 mice in ≥2 independent experiments). (e and f) Frequency of D-Red⁺ cells among αβ CD8⁺ (e) and γδ (f) T cells immediately, 7 days or 28 days after photoconversion of mesenteric LN in VHD mice (representative of ≥2 mice in ≥2 independent experiments).