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. 2018 Jun 12;8:8930. doi: 10.1038/s41598-018-27208-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) At day 14, brain metastases appeared as regions of hyperintensity by MRI, and (B) regions of BLI signal in the brain and body. (C) Mice with a large MFP tumor had significantly more brain metastases than mice without a primary tumor. (D) Mice with a large MFP tumour had significantly more total brain tumor burden than mice without a primary tumor. (E,F) Similarly, mice with a large MFP tumour had significantly more BLI signal in both the brain and the body compared to control mice. Data is presented as mean +/− SD. *Indicates p < 0.05; **indicates p < 0.01.