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. 2018 Jun 12;8:8926. doi: 10.1038/s41598-018-27281-9

Figure 1.

Figure 1

Expression of P2X7R and Panx1 in INS-1E cells. (a) Representative gel of P2X7 and Panx1 mRNA expression. (b) Representative Western blot of P2X7R and Panx1 expression in INS-1E cells grown in increasing glucose concentrations. Loading control was β-actin. The images (in a and b) are cropped from original full-length gels and blots that are shown in the supplementary material. (c,d) Western blot quantification of P2X7R and Panx1 (isoform A and D) expression in INS-1E cells grown in increasing glucose concentrations. The ratio of protein (P2X7R or Panx1) to β-actin were normalized to 2.8 mM glucose. Data represent means ± s.e.m. and n denotes the number of independent experiments and significant differences *p < 0.05 and **p < 0.01 are indicated.