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. 2018 Jun 12;8:8926. doi: 10.1038/s41598-018-27281-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolocalization of P2X7R and Panx1 in INS-1E cells, rat and mouse pancreas. Overlay images of INS-1E cells stained for P2X7R (a–d) and Panx1 (e–h) (green), insulin (red) and nuclei were stained with DAPI (blue). Co-localization graphs (d,h) show co-localization of P2X7R or Panx1 and insulin in corresponding images to the left (c,g). Person’s coefficient was x and y. Arrows indicate localization of the two proteins in the plasma membrane. Rat pancreas (i,j) and mouse pancreas (k,l) was stained for P2X7R (i,k) or Panx1 (j,l) (green) as above. All bars are 25 µm. Images shown here are representative of 3–10 images per slide on 3–9 independent staining experiments.