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. 2018 Jun 12;8:8926. doi: 10.1038/s41598-018-27281-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of apyrase and AZ10606120 on glucose-induced calcium transients. (a) Increase in glucose (from 2.8 to 16.7 mM) induced calcium oscillations in many cells and these were reduced by introduction of apyrase (10 U/ml). BzATP (100 μM) had no, if any, effect while increase in K⁺ to 30 mM caused further increase in intracellular Ca²⁺. (c) Similar protocol as in (a) but instead of apyrase AZ10606120 (10 μM) was added to the bath. Oscillations in Ca²⁺ abated and Fura-2 ratio increased until medium was exchanged with fresh low glucose solution. (b, d) Bargraphs shows means ± s.e.m. of height of Ca²⁺ oscillations in 2.8 mM, 16.7 mM glucose with/without apyrase (b) or AZ10606120 (d). Numbers indicate the number of cells analyzed in 5 and 4 independent experiments, respectively. Significant differences, **p < 0.01, are indicated.