Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 12;8:8984. doi: 10.1038/s41598-018-27174-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Energy transfer rates in FRET and BRET modes. (a–c) Schemes of the estimation of energy transfer rates and radiative decay rates. The fluorescent (a) and bioluminescent (b) spectra of hyBRET sensors were fitted with the spectra of CFP, YFP, and RLuc8 (c) by the nlinfit function of MATLAB to estimate the parameters. (d) Overlay of the measured (blue) and fitted (red) spectra of hyBRET-ERK before and after TPA stimulation. Envelopes with light red show the 95% confidence interval in the fitted curves. The y-axis indicates the emission intensity normalized by the area of spectrum. (e) Summary of the energy flow absorbed by RLuc8. Rates of energy transfer and radiative decay are shown. E_RC, E_RY, and E_CY are the energy transfer rates from RLuc8 to CFP, RLuc8 to YFP, and YFP to CFP, respectively. ϕ_C, ϕ_Y, and ϕ_R are the quantum efficiencies of CFP, YFP, and RLuc8, respectively.