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. 2018 Jun 12;8:8977. doi: 10.1038/s41598-018-27119-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HTR cells grown as spheroids reveal marked increase in ES markers compared to monolayers. (a) Shows mRNA expression of ES markers OCT4 (C_t = 23.1), SOX2 (C_t = 22.1), NANOG (C_t = 25.2),) in HTR cells grown as spheroids as compared to monolayers on plastic surface (n = 5, *indicates p < 0.05). (b) Shows immunostaining for SOX2 and NANOG under the same conditions. Representative images are selected from 5 different experiments.