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. 2018 Jun 12;8:8977. doi: 10.1038/s41598-018-27119-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HTR spheroids contain bi-potential progenitors for differentiation into EVT and syncytialization pathways. HTR cells grown as monolayers did not stain for HLA-G protein while spheroid derived cell clumps cultured overnight on plastic show HLA-G protein expression (a). Similarly there was no immunostaining for CGB on monolayer cells grown on plastic whereas some cells within the spheroid express CGB (b).