Table 1.
Results obtained for the described LC-MS/MS method for determination of OG and TG in rats’ plasma & brain tissue.
| Item | Rats’ plasma | Rats’ brain tissue | ||
|---|---|---|---|---|
| OG | TG | OG | TG | |
| Regression Eq. | Y = 0.0005 × −0.0124 | Y = 0.0007 × −0.0284 | Y = 0.0005 + 0.0161 | Y = 0.0007 × −0.0081 |
| Correlation Co. | 0.9994 | 0.9992 | 0.9980 | 0.9983 |
| *LLOQ (ng/mL) | 50 | 50 | 50 | 50 |
| *LQC (ng/mL) | 120 | 120 | 120 | 120 |
| *MQC (ng/mL) | 1500 | 1500 | 1500 | 1500 |
| *HQC (ng/mL) | 2500 | 2500 | 2500 | 2500 |
| Accuracy (*RE) | −14.08 to +15.26 | −10.82 to +19.07 | −12.24 to +8.53 | −13.1 to +7.98 |
| Precision (*% RSD) | 14.22–14.64 | 13.07–13.73 | 8.62–12.53 | 7.7–14.04 |
| *ME | 85.99–88.74 | 90.63–98.55 | 89.44–99.66 | 91.77–100.92 |
| ME for IS | 84.54–89.16 | 92.49–94.61 | ||
| Recovery % | 87.99–91.03 | 77.36–92.52 | 55.29–89.52 | 58.90–91.55 |
| Recovery % for IS | 73.83–87.64 | 55.01–85.02 | ||
| Stability studies | R% (84.33–95.09) | R% (85.92–97.42) | R% (70.35–89.96) | R% (86.90–91.55) |
*Where LLOQ is the lower limit of quantification, LQC is the low quality control sample, HQC is the high quality control sample, RE is the accuracy relative error, % RSD is the percent relative standard deviation, ME is the matrix effect & IS (the internal standard).