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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Nat Protoc. 2018 Apr 5;13(5):875–898. doi: 10.1038/nprot.2018.007
Component Amount
pEP4 E02S ET2K 2.5 µg
pEP4 E02S EN2L 2.5 µg
pEP4 E02S EM2K 2.5 µg
pSimple-miR302/367 2.5 µg
HDR template
Plasmid OR 5 µg
ssODN 0.3 nmol
sgRNA plasmid 2 µg
EBNA1 mRNA 5 µg
Cas9-Gem mRNA 5 µg