Component | Amount |
---|---|
pEP4 E02S ET2K | 2.5 µg |
pEP4 E02S EN2L | 2.5 µg |
pEP4 E02S EM2K | 2.5 µg |
pSimple-miR302/367 | 2.5 µg |
HDR template | |
Plasmid OR | 5 µg |
ssODN | 0.3 nmol |
sgRNA plasmid | 2 µg |
EBNA1 mRNA | 5 µg |
Cas9-Gem mRNA | 5 µg |
Component | Amount |
---|---|
pEP4 E02S ET2K | 2.5 µg |
pEP4 E02S EN2L | 2.5 µg |
pEP4 E02S EM2K | 2.5 µg |
pSimple-miR302/367 | 2.5 µg |
HDR template | |
Plasmid OR | 5 µg |
ssODN | 0.3 nmol |
sgRNA plasmid | 2 µg |
EBNA1 mRNA | 5 µg |
Cas9-Gem mRNA | 5 µg |