Figure 2.

Activation of adenylyl cyclase (AC) and downstream protein kinase A (PKA) underlies the kainate-mediated facilitation of glutamate release in PF-PuC synapses. (A) Time-course of the effect of KA on eEPSC amplitude in cAMP-Rp treated slices. Inset shows representative traces showing that KA (3 μM) does not increase the amplitude of the eEPSCs in cAMP-Rp treated slices. (B) Inhibition of PKA by cAMP-Rp (100 μM) or H-89 (2 μM) and activation of AC by forskolin (30 μM) prevented the facilitatory action of KA. Inhibition of protein kinase c (PKC) with calphostin C (1 μM) has no effect on the KA enhancement of the eEPSC amplitude. The facilitatory effect of KA is not affected in slices treated with pertussis toxin. The number of slices (from two to three mice) is indicated in parenthesis at the top of each bar. Results are expressed as means ± SEM (**P < 0.01, ANOVA test).