Figure 3.

Facilitation of glutamate release mediated by presynaptic kainate receptor (KAR) activation requires an increase of Ca²⁺ in the cytosol at PF-PuC synapses. (A) Time-course of KA (3 μM) effect on eEPSCs amplitude in control condition (circles) and in slices treated with philanthotoxin (squares). (B) Quantification of modulation observed in (A). (C) Time-course of the effect of KA on eEPSCs amplitude in control slices (circles) and in thapsigargin-treated slices (squares). (D) In slices treated with thapsigargin or ryanodine, the increase of eEPSCs amplitude induced by KA is prevented. The number of slices (from two to three mice) is indicated in parenthesis at the top of each bar. Results are expressed as means ± SEM (**P < 0.01, Student’s t-test and ANOVA).